In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies.
نویسندگان
چکیده
The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd(22)-PEO(13) and PMOXA(20)-PDMS(54)-PMOXA(20) block copolymer vesicles. The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening.
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عنوان ژورنال:
- Angewandte Chemie
دوره 52 2 شماره
صفحات -
تاریخ انتشار 2013